SPINK3 TESP1 contraceptive protein–protein interaction receptor serine protease inhibitor Kazal type 3 sperm acrosome testicular serine protease 1. If the binding affinity between SPINK3 and TESP1 could be increased, the SPINK3-TESP1 association will be prolonged, which will be helpful in the development of a male contraceptive. The results predicted that SPINK3 and TESP1 had strong binding affinity, with a dock score of -430.70 and 14 hydrogen bonds as key active site residues. Sundar Balu is the Senior Director Global Market Development at Weiss North America, INC. Protein-protein docking was performed using HDOCK and the complex was validated by MDS. The modelled protein structures were validated and subjected to molecular dynamics simulation (MDS) using GROMACS v5.0.5. Furthermore, the 3D structures of SPINK3 and TESP1 were predicted by homology modelling (Schrodinger suite) using the crystal structure of pancreatic secretory trypsin inhibitor (PDB ID-1TGS) and human prostasin (PDB ID-3DFJ) as templates. To authenticate TESP1 as the receptor for SPINK3, sperm cells were incubated with TESP1 peptide antibody followed by determining the intracellular i concentration by flow cytometry using Fluo-3 AM as a calcium probe. Testicular serine protease 1 (TESP1) was identified as the receptor for SPINK3 by 2D gel electrophoresis coupled with western blot analysis.
We identified the SPINK3 receptor by using recombinant SPINK3 (rSPINK3). Identification of the SPINK3 binding partner becomes essential to develop a contraceptive that works by prolonging the binding of SPINK3 to the sperm acrosome.
However, under natural coitus, SPINK3 is removed from the mouse acrosome in the female reproductive tract, leading to successful fertilisation. Santhanam was not secretly getting married to Ashna Zaveri in Tirupati Ashna. Previously, SPINK3 was reported to suppress in vitro sperm capacitation. After Trisha's film, Sundar Balu sets out with Varalaxmi, Aishwarya Dutta, Ashna Zaveri, Subiksha for Kannitheevu. It has been shown to bind to the sperm acrosome and modify sperm activity by influencing the sub-cellular Ca2+ influx. Serine protease inhibitor Kazal type 3 (SPINK3) from mouse seminal vesicles is a Kazal-type trypsin inhibitor.
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